Yoshiki Ikeda1, Akira Iizuka1, Sakae Amagaya1,
Atsushi Ishige1, Yasuhiro Komatsu1 and Eiichi Fujihira2
1CentralResearch Laboratories, Tsumura & Co., 3586, Yoshiwara,
Amiﾐmachi, Inashikiﾐgun, Ibaraki 300ﾐ1192, Japan
2Departmentof Pharmacotherapy, Hokkaido College of Pharmacy,
7ﾐ1, Katsuraokaﾐmachi, Otaru, Hokkaido 047ﾐ0264, Japan
Abstract: We investigated the antiﾐallergic effect of maoﾐbushiﾐsaishinﾐto
(MBS) on the typeI allergy model in mice. When MBS was administered orally
at a dose of 0.5 or 1.0 g/kg, edema of the footpad, the amount of plasma
IgE and the ratio of eosinophilic leukocytes in peritoneal exudate cells
were all doseﾐrelatedly suppressed. Moreover to investigate the antiﾐtypeI
allergic mechanisms of MBS, enzymeﾐlinked immunosorbent assay was performed
to determine the interleukin (IL)ﾐ4, ILﾐ5 and interferon (IFN)ﾐgamma production
from splenocytes that were stimulated by pokeweed mitogen for 48 h. In addition,
we assayed IgE production from splenic Bcells stimulated with the lipopolysaccharide
and ILﾐ4 for 7 days. MBS inhibited the ILﾐ4 and IFNﾐgamma production, but
ILﾐ5 and IgE production were not affected. Thus possibly, the inhibition
of ILﾐ4 production may partially be involved in the expression of the antiﾐtypeI
allergic effects of MBS.
Keywords: Maoﾐbushiﾐsaishinﾐto, Antiﾐallergy, Interleukinﾐ4, IgE,
Spleen cell
Copyrightｩ The Japanese Pharmacological Society 2000
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