Jpn. J. Pharmacol. 82 (1), 71-73 (2000)

Perivascular Purinergic NerveﾐInduced Vasoconstrictions
in Canine Isolated Splenic Arteries

XiaoﾐPing Yang and Shigetoshi Chiba*


Department of Pharmacology, Shinshu University School of Medicine, Matsumoto 390ﾐ8621, Japan
*To whom correspondence should be addressed.

Abstract: We tried to induce selective perivascular purinergic nerve stimulation in isolated canine splenic arterial preparations, using the cannula insertion method. Under the conditions of periarterial electrical stimulation (ES), i.e., trains of 1, 3 and 10 pulses, 1ﾐms pulse duration and 10ﾐV amplitude at 1 Hz, monophasic vasoconstriction was consistently induced. The ESﾐinduced vasoconstriction was not influenced by prazosin in doses that completely inhibited noradrenalineﾐinduced vasoconstrictions, but it was suppressed by alpha,betaﾐmethylene ATP, a P2X purinoceptor desensitizer. Thus, it is indicated that a selective purinergic transmitter release is readily obtained in the isolated splenic arterial preparation.

Keywords: Dog splenic artery, Perivascular electric stimulation, P2X Purinoceptor