Jpn. J. Pharmacol. 83 (2), 157-160 (2000)

Acceleration by KW-5092 of Intestinal Motility Associated
With Acetylcholine Release In Vivo

Noriaki Makimoto¹, Akira Yamazaki², Hiroyuki Kobayashi², Nobuyuki Kishibayashi², Kenji Ohmori²,
Akira Furuichi¹, Takashi Kanematsu¹ and Kohtaro Taniyama^3,*


Departments of ¹Surgery II and ³Pharmacology, Nagasaki University School of Medicine, Nagasaki 852-8523, Japan
²Departmentﾊof Pharmacology, Pharmaceutical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Shizuoka 411-8731, Japan
*ﾊTo whom correspondence should be addressed.

Abstract: Effect of KW-5092 ({1-[2-[[[5-(piperidinomethyl)-2-furanyl]methyl]amino]ethyl]-2-imidazolidinylidene}propanedinitrile fumarate) on intestinal motility and release of endogenous acetylcholine (ACh) were measured simultaneously in the small intestine of anesthetized dog using the in vivo microdialysis method. Intraarterial and intravenous administrations of KW-5092 accelerated the intestinal motility and increased dialysate ACh concentrations. These KW-5092-induced responses paralleled the increase in blood concentration of KW-5092. Thus, the acceleration of intestinal motility by KW-5092 was found in vivo to be associated with an increase in ACh release from the intestinal cholinergic neurons.

Keywords: Intestinal motility, Acetylcholine release, In vivo microdialysis