Yukio Sugimoto, Yoshinori Iba, Keiko Utsugi and Chiaki Kamei*
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Okayama
University, Okayama 700-8530, Japan
*ﾊTo whom correspondence should be addressed.
Abstract: We examined influences of certain antibiotics on the
release of chemical mediators from isolated rat peritoneal mast cells in
vitro. Isolated peritoneal mast cells were obtained from male Wistar strain
rats. Everninomicin (0.06-1.2ﾊmg/ml), vancomycin (0.05-1.0ﾊmg/ml), teicoplanin
(0.07-1.4ﾊmg/ml) and concanavalinﾊA (0.01ﾊmg/ml) were used. Isolated mast
cells were incubated in the presence of various concentrations of the test
compound at 37｡C for 10ﾊmin. Histamine contents of the supernatant and cell
pellet were measured by an automated fluorometric method. ProstaglandinﾊD2
(PGD2) contents of the supernatant were determined by enzyme
immunoassay. Everninomicin (0.06-1.2ﾊmg/ml) had no influence on histamine
and PGD2 release from mast cells. On the other hand, vancomycin
significantly released both histamine (0.5 and 1.0ﾊmg/ml) and PGD2
(1.0ﾊmg/ml) from mast cells, but vancomycin did not affect concanavalinﾊA-induced
histamine and PGD2 release. Teicoplanin (more than 0.07ﾊmg/ml)
significantly stimulated histamine and PGD2 release from mast
cells and it also significantly potentiated concanavalinﾊA-induced histamine
and PGD2 release. These results suggest that everninomicin causes
no chemical mediator release from mast cells, different from vancomycin
and teicoplanin.
Keywords: Everninomicin, Vancomycin, Teicoplanin, Histamine release,
Prostaglandin D2 release