Shinji Teramoto1*, Takeo Ishii2, Takeshi Matsuse3
and Yoshinosuke Fukuchi4
1Department of Internal Medicine, San-no Hospital, Tokyo (Medical
Research Center, International University of Health and Welfare), 8-5-35
Akasaka, Minato-ku Tokyo 107-0052, Japan
2Department of Geriatric Medicine, Tokyo University Hospital,
Tokyo 113-8655, Japan
3Department of Pulmonary Medicine, Yokohama City University Medical
Center, Yokohama 232-0024, Japan
4Department of Respiratory Medicine, Juntendo University School
of Medicine, Tokyo 113-8421, Japan
*Corresponding author.ﾊﾊFAX:+81-3-3404-3652
E-mail: shinjit-tky@umin.ac.jp
Abstract: Because the features and kinetics of adeno-associated virus
(AAV)-mediated gene transfer to endothelial cells (EC) are yet to be ultimately
determined, we tested variables pertinent to the efficiency of AAV-mediated
gene transfer to bovine aortic endothelial cells (BAEC). The variables with
AAV vectors were compared with the better characterized adenovirus (Ad)
vectors. There is a dose-response relationship between multiplicity of infection
(moi) of AAV or Ad vectors and transduction efficiency in BAEC. The higher
moi of AAV vectors achieved more than 80% of transduction efficiency in
cultured BAEC. AAV and Ad vectors showed an incubation-time-dependent increase
in transduction efficiency of LacZ gene to the BAEC up to 12ﾊh of vector
exposure. Although the similar kinetics of transduction efficiency of LacZ
gene to BAEC was found in both vectors, the duration of gene expression
was longer in AAV vector than that in Ad vectors in vitro. These results
indicate that AAV-vector is efficient for gene transfer to EC, and higher
moi of vectors or a longer period exposure of vectors to EC can facilitate
efficient transduction of a foreign gene into cultured EC. For the duration
of gene expression, the AAV vectors may be better than Ad vectors.
Keywords: Endothelial cell, Gene transfer, Adeno-associated virus vector,
Adenovirus vector
Copyrightｩ The Japanese Pharmacological Society 2000
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