Hitoshi Ishibashi, Satoshi Okuya, Hideaki Shimada and Kazuo Takahama*
Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences,
Kumamoto University, 5-1 Oe-Honmachi, Kumamoto 862-0973, Japan
*Corresponding author.ﾊﾊFAX:+81-96-371-4334
E-mail: takahama@gpo.kumamoto-u.ac.jp
Abstract: The effect of a synthetic estrogen, diethylstilbestrol
(DES), on kainate-induced currents was investigated in the hippocampal CA1
pyramidal neurons acutely dissociated from the mice using the nystatin-perforated
patch-clamp recording configuration under voltage-clamp conditions. DES
inhibited the current evoked by 100ﾊmM kainate
in a concentration-dependent manner with a half-maximum inhibitory concentration
of 8.8ﾊmM. The action of DES was voltage-independent.
Since DES produced a suppression of the maximum response of the kainate
concentration-response curve, the inhibition by DES of the kainate-induced
current appears to be non-competitive.
Keywords: Nystatin-perforated patch-clamp recording, Diethylstilbestrol,
Environmental endocrine disrupter
Copyrightｩ The Japanese Pharmacological Society 2000
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