Tomohisa Hirai1, Hiromichi Tsuru2,*, Noriaki Tanimitsu1,
Masaya Takumida1, Hiroshi Watanabe1, Koji Yajin1
and Masashi Sasa3
1Department of Otorhinolaryngology and 3Department
of Pharmacology, Hiroshima University School of Medicine, 1-2-3 Kasumi,
Minami-ku, Hiroshima 734-8551, Japan
2Department of Pharmacology, Toho University School of Medicine,
5-21-16 Ohmori-nishi, Ohta-ku, Tokyo 143-8540, Japan
*Corresponding author.ﾊﾊFAX:+81-3-5493-5413
E-mail: htsuru@med.toho-u.ac.jp
Abstract: The effect of hydrogen peroxide (H2O2)
on guinea pig nasal mucosa vasculature was studied by in vitro assay. H2O2
elicited relaxation of guinea pig nasal mucosa strips precontracted with
phenylephrine in a concentration-dependent manner. The relaxant response
to H2O2 was abolished in the presence of catalase.
Preincubation of the strips with NG-nitro-L-arginine methyl ester or methylene blue significantly attenuated
the relaxant responses elicited by H2O2. Fluorescence
caused by DAF-2 DA, a fluorescence indicator for nitric oxide, was observed
along the nasal mucosa vasculature in response to H2O2.
These results suggest that H2O2 induced relaxation
of the guinea pig nasal mucosa vasculature and that this relaxation is mediated
by the NO/cGMP pathway.
Keywords: Nasal mucosa vasculature, Hydrogen peroxide, Nitric oxide
Copyrightｩ The Japanese Pharmacological Society 2000
[Back to TOC]