Jpn. J. Pharmacol. 85 (1), 105-108 (2001)

Guinea Pig Histamine N-Methyltransferase: cDNA Cloning and mRNA Distribution

Junichi Kitanaka¹, Nobue Kitanaka¹, Tohru Tsujimura², Michiko Kakihana², Nobuyuki Terada² and Motohiko Takemura^1,*

Departments of ¹Pharmacology and ²Pathology I, Hyogo College of Medicine, 1-1 Mukogawa-cho, Nishinomiya, Hyogo 663-8501, Japan
*Corresponding author. FAX: +81-798-45-6332
E-mail: takemura@hyogomc.kugi.kyoto-u.ac.jp

Abstract: We report here the isolation of histamine N-methyltransferase (HMT) cDNA from the guinea pig brain by the polymerase chain reaction on the basis of nucleotide sequences of rat and human counterparts. Guinea pig HMT consists of 292 amino acids, with homologies of 75.6% and 79.1% to rat and human HMT, respectively. Northern blotting analysis indicated that the 1.6-kb guinea pig HMT transcript was expressed at various levels in different tissues at the following relative abundance: jejunum, brain > lung, spleen, stomach > liver, kidney. HMT mRNA localized throughout　the jejunum, and it was mainly expressed in epithelial cells and in Auerbach's plexus.

Keywords: Histamine N-methyltransferase, mRNA, Localization