Muneshige Kaibara*, Yoshihisa Nagase, Osamu Murasaki, Yasuhito Uezono, Yoshiyuki Doi and Kohtaro Taniyama
Department of Pharmacology, Nagasaki University School of Medicine, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan
*Corresponding author. FAX: +81-95-549-7048, E-mail: mkaibara@alpha.med.nagasaki-u.ac.jp
Abstract: In native Xenopus oocytes, injection of guanosine 5'-O-(3-thiotriphosphate) (GTPgS) (30 mM, 5 nl) did not induce Cl− current in 11 out of 22 oocytes. Injection of increased concentration of GTPgS (100 mM, 5 nl) into the oocytes induced Cl− currents in 16 out of 17 oocytes; however, the size of the induced currents was extremely varied. In oocytes overexpressing Gqa, GTPgS (30 mM, 5 nl) faithfully evoked Ca2+-activated Cl− currents. These results indicate that heterogeneous expression of Gqa in Xenopus oocytes provides a useful system for studying the functional roles of Gqa in regulating cellular events.
Keywords: Gq, GTPgS, Oocyte
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