Yoshiko Kawai and Munekazu Gemba
Division of Pharmacology, Osaka University of Pharmaceutical Sciences, Nasahara, Takatsuki, Osaka 569, Japan
Abstract: We investigated the effects of a phosphodiesterase inhibitor and dibutyryl cAMP (dBcAMP) on the cell injury induced by cephaloridine (CER) in an established renal epithelial cell LLC-PK1. CER increased the leakage of lactate dehydrogenase (LDH) from LLC-PK1 cells to the medium and the level of lipid peroxidation in the cells. 3-Isobutyl-1-methylxanthine, a phosphodiesterase inhibitor, increased cAMP content in LLC-PK1 cells and ameliorated the increase in LDH leakage induced by CER. dBcAMP reduced the cell injury induced by CER. Our results suggest that a signalling pathway of cAMP protects against CER-induced renal cell injury, which is probably due to generation of oxygen radicals.
Keywords:
cAMP and oxidative stress, Renal cell injury, Cephaloridine nephrotoxicity