Jpn. J. Pharmacol. 74 (2), 139-146 (1997)
Nicotinic Agonist Modulation of Neurotransmitter Levels in the Rat Frontoparietal
Cortex
Kathleen L. Summers (1), William R. Kem (2,*) and Ezio Giacobini (1,#)
(1) Department of Pharmacology, Southern Illinois University, Springfield,
IL 62794-9230, USA
(2) Department of Pharmacology and Therapeutics, College of Medicine, University
of Florida, Gainesville, FL 32610-0267, USA
(*) To whom correspondence should be addressed.
Present address: Dept. of Geriatrics, University of Geneva, Medical School,
Route de Mon-Idee, CH-1226 Thonex-Geneva, Switzerland
Abstract: Anabaseine is a naturally occurring toxin that stimulates
a variety of neuronal and muscleÅ@nicotinic receptors. GTS-21 [3-(2,4-dimethoxybenzylidene)anabaseine],
an anabaseine derivative, selectively stimulates alpha7-containing nicotinic
receptors. Here we report the first in vivo study of the effects of these
two nicotinic agonists on cortical extracellular acetylcholine (ACh), dopamine
(DA), norepinephrine (NE) and serotonin (5-HT) levels, measured with a microdialysis
probe placed within the frontoparietal cortex inÅ@the absence of a cholinesterase
inhibitor. At 3.6 micromol/kg, s.c., anabaseine increased cortical ACh and
NEÅ@above baseline values without significantly affecting DA and 5-HT. The
ACh and NE elevations were inhibited by i.p. pre-administration (4.9 micromol/kg)
of the nicotinic antagonist mecamylamine (Mec). In contrast, GTS-21 (3.6
micromol/kg, s.c.) significantly increased NE and DA without affecting ACh
and 5-HT levels. Following Mec injection, GTS-21 increased ACh 25-fold and
5-HT 13-fold, while NE and DA levels were slightly decreased in comparison
with GTS-21 alone. We suggest that at the dose used, Mec may preferentially
block high affinity nicotinic receptors which normally provide an inhibitory
influence uponÅ@ACh release, thereby permitting expression of the complete
stimulatory effect of GTS-21 on neuronal alpha7-receptors. GTS-21 and other
receptor subtype-selective nicotinic agonists should be helpful in clarifying
theÅ@roles of particular nicotinic receptors in modulating cortical neurotransmitter
levels.
Keywords: Acetylcholine, Alzheimer's therapy, Mecamylamine, Microdialysis,
Nicotinic agonist
Copyright© The Japanese Pharmacological Society 1997
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