Yoshihisa Kitamura (1), Tadashi Kosaka (1), Shun Shimohama (2), Yasuyuki
Nomura (3) and Takashi Taniguchi (1,*)
(1) Department of Neurobiology, Kyoto Pharmaceutical University, Kyoto
607, Japan
(2) Department of Neurology, Faculty of Medicine, Kyoto University, Kyoto
606, Japan
(3) Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokkaido
University, Sapporo 060, Japan
(*) To whom correspondence should be addressed.
Abstract: In human neuroblastoma SH-SY5Y cells, treatment with
immunosuppressants such as FK506, cyclosporin A or rapamycin for 4 days
induced the enhancement of the 27-kDa Bcl-2alpha protein level. Among immunosuppressants,
rapamycin has most potency. Treatment with herbimycin A or wortmannin also
enhanced Bcl-2 expression, but the BB type of platelet-derived growth factor
decreased the level. These results suggest that Bcl-2 expression is probably
regulated by the cascade of tyrosine kinase, phosphatidylinositol 3-kinase
and rapamycin-sensitive p70 S6-kinase in human neuroblastoma SH-SY5Y cells.
Keywords: Bcl-2, Rapamycin, p70 S6-kinase