Hitoshi Kontani, Masumi Jinkawa, Chisato Shiraoya and Akiko Nagashima
(#)
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokuriku
University, Kanagawa-machi, Kanazawa 920-1181, Japan
(#) Present address: Tobishi Yakuhin Kogyo Co., Oume, Tokyo 198-0025, Japan
Abstract: The effects of KRN2391 (N-cyano-N'-(nitroxyethyl)-3-pyridine
carboximidamide methanesulfonate), which possesses ATP-sensitive potassium
(K+) channel opening (KCO) activity and nitrate activity; Ki1769
(N-cyano-N'-(phenylethyl)-3-pyridinecarboximidamide methanesulfonate),
which possesses only KCO activity; and nitroglycerin (NG) were determined
on the motility of the ureter, urinary bladder and urethra of rats. Bladder
contraction was induced by infusion of fluid into the bladder of conscious
rats and recorded on a cystometrogram. KRN2391 and Ki1769 (both 0.3 mg/kg,
i.v.) prolonged the micturition interval immediately after the injection,
but NG (5 mg/kg, i.v.) did not. Peristaltic movement of the ureter, recorded
in anesthetized rats, was inhibited by i.v. injection of KRN2391 and Ki1769
(both 0.03 mg/kg). However, when NG, NaNO2, N-nitro L-arginine
methylester and methylene blue were applied directly to the ureter, no change
in movement of the ureter was detected. KRN2391 (0.03 mg/kg, i.v.) and Ki1769
(0.3 mg/kg, i.v.) reduced the resistance to fluid infusion through the urethral
lumen in anesthetized rats, whereas NG (0.5 mg/kg, i.v.) only reduced this
resistance transiently. These results indicate that KCO activity had an
inhibitory effect on the motility of the ureter, bladder and urethra. On
the other hand, nitrate activity had an inhibitory effect on urethral tonus,
corresponding to that induced by KCO activity.
Keywords: K+ channel opener, Nitroglycerin, Rat cystometrogram,
Ureteral peristaltic movement, Urethral resistance