Koji Tomiyama, Eisuke Nishio and Yasuhiro Watanabe
Department of Pharmacology, National Defense Medical College, 3 - 2 Namiki,
Tokorozawa, Saitama 359 - 0042, Japan
Abstract: As we reported previously, both wortmannin and lovastatin
inhibit the differentiation in 3T3-L1 cells when these drugs were applied
during the insulin-induced cell differentiation. In the present study, 3T3-L1
cells were treated with wortmannin and simvastatin after the completion
of the insulin-stimulation, and differentiation was found to be significantly
decreased by these drugs. This suggests intracellular signaling pathways
play roles in the differentiation of 3T3-L1 cells even after the completion
of the insulin-stimulation and also suggests that not only the early phase
(day 0 to day 2) but also the late phase (day 2 to day 4) of differentiation
is important for the differentiation of the cells.
Keywords: Simvastatin, Wortmannin, Adipocytic differentiation