Takashi Fujita, Toru Meguro, Nobuo Izumo, Chigusa Yasutomi, Ryo Fukuyama,
Hiromichi Nakamuta and Masao Koida*
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Setsunan
University, Nagaotogecho 45-1, Hirakata 573-0101, Japan
*Corresponding author. FAX: +81-72-866-3108
E-mail: koida@pharm.setsunan.ac.jp
Abstract: ATDC5 cells were employed to examine how inorganic phosphate
(Pi) influences chondrocytic bone formation. 1) Pi (3-30 mM) plus ascorbic
acid (50 mg/ml) dose-dependently accelerated proliferative
differentiation and mineralization of ATDC5. 2) Northern blot analysis revealed
that 10 mM Pi suppressed expression of type II collagen and PTH (parathyroid
hormone) /PTH-related peptide (PTHrP) receptor, while it accelerated type
X collagen expression. 3) Pi (3-30 mM) dose-dependently increased luciferase
activity in the cells transfected with 3000 bp type X collagen promoter
fused to the luciferase gene. The results suggest a regulatory role of Pi
in endochondral osteogenesis.
Keywords: Phosphate, ATDC5, Endochondral osteogenesis
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